DISSERTAÇÃO DE MESTRADO - PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS DA SAÚDE
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Item AÇÃO PROFILÁTICA DO RANELATO DE ESTRÔNCIO NAS ALTERAÇÕES MORFOLÓGICAS DE OSTEOARTRITE EXPERIMENTAL.(Universidade Federal do Maranhão, 2018-07-18) CARVALHO, Heetor Campora Oliveira; CARTÁGENES, Maria do Socorro de Sousa; 407.888.653-15; http://lattes.cnpq.br/3013333572719007; GARCIA, João Batista Santos; 135.570.488-02; http://lattes.cnpq.br/0424234103760462; GARCIA, João Batista Santos; http://lattes.cnpq.br/0424234103760462; VIEIRA, Érica de Brandão de Moraes; http://lattes.cnpq.br/2265220151524135; VASCONCELOS, José Wanderley; http://lattes.cnpq.br/8606202391209961; GUERRA, Rosane Nassar Meireles; http://lattes.cnpq.br/2316192786452127Introduction: Osteoarthritis (OA) is a multifactorial disease as a result of several and complex mechanisms interacting to cause joint damage. There is no cure for OA, but progression can be controlled. Many drugs can act to decrease manifestations of OA, such as strontium ranelate (RnSr), but its preventive effect on joint injuries has not yet been demonstrated. Aim: To evaluate the prophylactic effect of RnSr in an experimental model of osteoarthritis in rats' knees. Methods: The animals were divided into 3 groups, called Prophylactic, Control and Naïve, with 6 mice each. Prophylactic group received RnSr at the dose of 250 mg/kg orally for 07 days until OA induction; Control group received 0.9% saline solution also orally and for the same period, being submitted to OA induction; and Naïve group had only standard feeding, not submitted to OA induction. Twenty-nine days after OA induction animals were euthanized, knee Xray was performed, knee joint cartilage and synovial membrane were collected. Results: Radiographic images showed the greatest changes in Control group, with statistical significance (p<0.05). Regarding to articular cartilage changes no alteration were found in Naïve group, however, there was a significant change in Control group comparing to Prophylactic group (p<0.05). Synovial membrane was not affected by the treatment, since the results were similar in all groups (p>0.005) In the evaluation of the synovial membrane, no statistical difference was observed between the study groups (p>0.05). Conclusion: Results indicate that RnSr is a drug with potential prophylactic effect on OA, decreases changes in the articular cartilage and the degenerative pattern of knee joints.Item ANSIEDADE E DEPRESSÃO EM PACIENTES SUBMETIDOS À CIRURGIA DE REVASCULARIZAÇÃO DO MIOCÁRDIO.(Universidade Federal do Maranhão, 2016-10-05) LULA, Josimary Lima da Silva; NINA, Vinicius José da Silva; 427.880.483-00; http://lattes.cnpq.br/5148313598301706; NINA, Vinicius José da Silva; 427.880.483-00; http://lattes.cnpq.br/5148313598301706; CARVALHO, Cristianne Almeida; http://lattes.cnpq.br/5714971389284118; SIMÕES, Vanda Maria Ferreira; http://lattes.cnpq.br/4024829764707677; CARVALHO, Alexandre Guilherme Ribeiro; http://lattes.cnpq.br/7062148392179283; LESSA, Jadir Machado; http://lattes.cnpq.br/1704453729588865Background: Coronary artery bypass grafting (CABG) is a procedure performed in patients with coronary artery disease (CAD). Perioperatively this type of surgery, patients the most diverse emotions and feelings are exposed, among them anxiety and depression. Objective: To evaluate emotional aspects of patients undergoing coronary artery bypass surgery. Methods: This was a cross-sectional study conducted in São Luís - MA with 41 adult patients undergoing CABG. Anxiety was assessed by the Beck Anxiety Inventory (BAI) and depression by Beck Depression Inventory (BDI). Results: There were more often male (60.98%), under 65 (53.66%), average of 64.04 ± 9.02 years, incomplete primary education (29.27%), family income between one and two minimum wages, body mass index (BMI) (<25) (43.90%) and patients with hypertension (78.05%). The anxiety in the preoperative period was present in 60.98%, increasing postoperative (75.61%) and maintained the same frequency as the pre ICU (60.98%) with statistically significant differences for sex (p=0.032) in the postoperative period. Depression showed frequency of 72.50%, 82.93% and 82.93%, respectively preoperatively, post and ICU, in which the income was significant postoperative (p = 0.022) and in the ICU (p = 0.011), BMI (p = 0.0047) and hypertension (p=0.041) before. Conclusion: These data demonstrated the need to incorporate new assistance strategies to worry about the psychosocial dimensions of patients, thereby providing early diagnosis and treatment of anxiety disorders and depression.Item Atividade antiulcerogênica do extrato aquoso das folhas de Psidium guineense Swartz(Universidade Federal do Maranhão, 2016-05-13) SOUSA, Ana Kely Araújo de; CALDAS, Germana Freire Rocha; 922.122.713-87; BORGES, Marilene Oliveira da Rocha; 227.298.602-15Psidium guineense Swartz (Myrtaceae) is a popular shrub commonly as araçá, araçá do campo, araçá vedaleiro, araçá mirim, araçá pedra ou araçazinho, and is widely distributed in Brazil, taking place in the Amazon, Caatinga, Cerrado and Atlantic Forest. Its barks and roots are used commonly in the treatment of gastrointestinal disorders such as colic, diarrhea, gastroenteritis and gastritis. The phytochemical research reveals that the species is rich in flavonoids and tannins, classes of compounds that have shown potential antiulcerogenic activity. Based on their phytochemical characteristics and in popular usage, the aim of this study was to investigate the antiulcerogenic activity of aqueous extract of Psidium guineense (EAPg) and possible mechanisms involved in this activity. The extract was prepared from the powder by aqueous extraction of the leaves at 60 °C. In acute toxicity, EAPg was administered orally in a single dose (5g/kg). The antiulcer activity of EAPg (125, 250 and 250 mg/kg) was evaluated using models of gastric ulcer induced by etanol 75% (10 mL/kg) or indomethacin (30 mg/kg). Were determined baseline antisecretory activity by the method of pyloric ligation, the involvement of nitric oxide and sulfhydryl compounds, and quantified the levels of gastric mucus and sulfhydryl compounds. The anti-Helicobacter pylori activity was determined by in vitro assay. No signs of toxicity or death was recorded after oral administration of EAPg at a dose of 5.0 g/kg. Oral administration of EAPg (125, 250 and 500 mg/kg) was effective in protecting the gastric mucous membrane, reducing by 45.0, 88.0 and 93.0% gastric lesions induced by ethanol, respectively. Furthermore, EAPg reduced by 53.0, 79.0 and 94.0% gastric ulcer induced by indomethacin, respectively. EAPg (250 mg/kg) increased the pH and reduced the total acidity, but did not change the volume of gastric secretion, suggesting an absence of antisecretory effect. The antiulcerogenic activity EAPg is not related to nitric oxide, but is dependent on the presence of sulfhydryl compounds. The prior administration of EAPg increased basal levels of mucus 89,4% and 78,9% sulfhydryl compounds in the gastric mucosa, thus confirming their cytoprotective activity. The EAPg showed no bactericidal activity against Helicobacter pylori, having a minimum inhibitory concentration (MIC) 2000 mg/mL. The results showed that the aqueous extract of Psidium guineense has antiulcer activity. In addition, they contribute to a better understanding of the species and to validate the use of this species in the treatment of gastrointestinal disordersItem Atividade de óleo essencial de Lippia gracilis e seus monoterpenos majoritários associados à carrapaticidas sintéticos em Rhipicephalus microplus (Acari: Ixodidae)(Universidade Federal do Maranhão, 2017-04-04) MILHOMEM, Marlise Neves Milhomem; COSTA JUNIOR, Lívio Martins; 819.183.563-00The Rhipicephalus microplus tick is considered one of the biggest problems for cattle breeding in Brazil. The continuous use of synthetic acaricides to control this tick has increased the frequency of resistance. Work using extracts and plant oils, are shown to be promising in the control of R. microplus. Lippia gracilis is a plant rich in essential oil, presenting major compounds which have a proven carrapaticide effect. The objective of this work was to evaluate the effect of the association between synthetic carrapaticides and natural compounds on resistant and susceptible synthetic Rhipicephalus microplus larvae. Immersion tests of R. microplus larvae were carried out using synthetic carrapaticides and essential oils (OE) of L. gracilis and their major compounds. First the lethal concentration to 50% (LC50) of the larvae was found for each of the isolated compounds. Associations of each of the natural compounds associated with a synthetic carrapaticides. In the obtained results it is observed that there was an antagonistic effect in the associations with one of the synthetic carrapaticides used, however in the associations with the other carrapaticide a strong synergic effect is observed, the same occurred for the two strains. We have shown that associations of natural compounds with synthetic carrapaticides may be a promising alternative in the control of R. microplusItem ATIVIDADE LARVICIDA SOBRE Aedes aegypti L. (Culicidae) E COMPOSIÇÃO QUÍMICA DO ÓLEO ESSENCIAL DE PARTES AÉREAS DE Baccharis trimera (Less) D.C.(Universidade Federal do Maranhão, 2018-05-07) SILVEIRA, Daniella Patrícia Brandão; COUTINHO, Denise Fernandes; 515.551.073-20; http://lattes.cnpq.br/7346399893912346; COUTINHO, Denise Fernandes; 515.551.073-20; http://lattes.cnpq.br/7346399893912346; COSTA, Maria Célia Pires; http://lattes.cnpq.br/4515777673951926; CÂMARA, Adriana Leandro; http://lattes.cnpq.br/4437683029436623; AMARAL, Flávia Maria Mendonça do; http://lattes.cnpq.br/9334507801916334Mosquito-borne diseases still persist as an important global health problem. Aedes aegypti is one of the main vectors of diseases transmitted by mosquitoes, such as Yellow Fever, Dengue, Zika, Chikungunya; arboviruses that can lead to hemorrhagic shock, embryonic malformations, joint diseases and death Thus, one of the ways recommended by the World Health Organization for the control of arboviruses is the vector control, the mosquito Aedes aegypti. In this sense, there is a tendency to search for natural products that can be used as potential larvicides and insecticides. In this context, the objective of this work was to evaluate the chemical composition of Baccharis trimera (Less) DC and to verify if it presents activity against larvae of the Aedes aegypti vector and evaluate the toxicity of the product against non-target organizations. Plant material was obtained from the Multidisciplinary Center for Chemical, Biological and Agricultural Research - CPQBA of the State University of Campinas - UNICAMP. A sample was sent to the Herbarium of the Federal University of Maranhão, to confirm the species. The essential oil was extracted by hydrodistillation and the identification of the chemical compounds was obtained by Gas Chromatography coupled to Mass Spectrometry. It was possible to identify 90% of the substances present in the essential oil under study, being 10% composed of hydrocarbon monoterpenes, 14% of oxygenated monoterpenes, 24% of oxygenated sesquiterpenes and 45% of hydrocarbon sesquiterpenes. The essential oil in this study had as its major compounds spatulenol (18.45%), caryophyllene oxide (15.24%) and e-caryophyllene (9.18%). The evaluation of larvicidal activity was performed for the four larval stages (L1, L2, L3, L4) at six concentrations. Cytotoxicity tests against Artemia salina and Danio rerio were performed and the respective LC's50 were calculated. It is considered that the essential oil of Baccharis trimera is effective against larvae between the 1st and 4th stage of Aedes aegypti, it was obtained LC's50 equivalent to 38.8; 55.8; 89.7; 171.8 μg / mL for 1st, 2nd, 3rd and 4th stages, respectively. The oil may be considered toxic for Danio rerio, however it was non-toxic or of low toxicity in a toxicity test against Artemia salina.Item AVALIAÇÃO DA EFETIVIDADE DO PROGRAMA DE TRATAMENTO DA HEPATITE C CRÔNICA EM USUÁRIOS DA FARMÁCIA ESTADUAL DE MEDICAMENTOS EXCEPCIONAIS DO MARANHÃO (FEME).(Universidade Federal do Maranhão, 2011-10-18) TEIXEIRA, Fábio Gomes; FERREIRA, Adalgisa de Souza Paiva; 850.818.767-04; http://lattes.cnpq.br/6707376027989566; FERREIRA, Adalgisa de Souza Paiva; 850.818.767-04; http://lattes.cnpq.br/6707376027989566Cohort study using retrospective data to evaluate the effectiveness of the treatment program for chronic hepatitis C in users of Farmácia Estadual de Medicamentos Excepcionais do Maranhão (FEME) to determine the rate of sustained virologic response (SVR) and correlate it with demographic, clinical, laboratory, histological and virological features of patients and to identify the rates of discontinuation of treatment. We analyzed data from 256 patients treated for chronic hepatitis C in FEME for the period January 2005 to July 2009, being an SVR by intention to treat 57%. Males predominated (66%). The mean age was 52.5 years, with a predominance of non-white individuals in relation to whites. Genotype 1 was the most common (77%) and 150 (58.6%) patients had viral load above 400,000 UI/ml. With respect to the treatment regimen, pegylated interferon associated with ribavirin was used by 80.5% of patients, the rate of discontinuation of treatment of 13,3%. They were identified as factors independently associated with SVR: white, non-cirrhotic, have not a genotype 1 and viral load below 400,000 IU / ml. These findings demonstrate the effectiveness of treatment provided by FEME, which makes it possible to cure most patients, preventing progression to end-stage liver disease and its disastrous consequences. The factors associated with SVR have seen in other studies, leads us to believe that the results are reliable and that the program is fulfilling the role it has set itself.Item Caracterização de modelo de imunossupressão com ciclofosfamida em camundongos portadores ou não de tumor de ehrlich(Universidade Federal do Maranhão, 2019-04-24) ALBUQUERQUE, Paula Sibelly Veras; REIS, Aramys Silva dos; 017327953-80; http://lattes.cnpq.br/1040580590566490; NASCIMENTO, Flávia Raquel Fernandes do; 488271693-34; http://lattes.cnpq.br/9073277157401960; NASCIMENTO, Flávia Raquel Fernandes do; 005914953-17; http://lattes.cnpq.br/9073277157401960; Reis, Aramys Silva dos; http://lattes.cnpq.br/1040580590566490; FALCAI, Angela; http://lattes.cnpq.br/9374112086158829; LEAL, Plínio da Cunha; http://lattes.cnpq.br/2150178332757393; SANTOS, Ana Paula Silva de Azevedo dos; http://lattes.cnpq.br/8224124082144965Cyclophosphamide is a drug widely used in the treatment of cancers. Due to its non-specific antiproliferative action, it also acts as an immunosuppressant, an effect that is present in patients treated by this drug. In this context, it is opportune to develop preclinical models of immunosuppression concomitant with the experimental ones of cancer, for several purposes. Thus, the objective of this study was to characterize a model of immunosuppression with cyclophosphamide in mice with or without Ehrlich tumor. For this, male mice of the Swiss strain, aged 3 to 4 months, received in the right ear 5x105 Ehrlich tumor cells or PBS (20μL). From the second day after the inoculum, the animals received cyclophosphamide (25mg/kg) daily, intraperitoneally, for 10 days. Animals and feed were weighed at the beginning and at the end of treatment. On the twelfth day after the inoculum, the animals were euthanized and the ears were removed for macroscopic and histological analysis. In addition, the cells of the blood, peritoneum, bone marrow and spleen were quantified. Splenic immunophenotyping and evaluation of hydrogen peroxide (H2O2) were also performed. Initially the antitumor effect of cyclophosphamide was confirmed in the model used and the reduction of inflammatory infiltrate and necrosis in the ears with tumor was confirmed. As an immunosuppressive effect, this drug caused reduction of feed intake, animal weight, leucopenia, medullary aplasia and reduced the number of splenocytes. Cyclophosphamide, alone, induced a decrease in the number of B lymphocytes in the spleen. However, in animals with tumor, cyclophosphamide induced a decrease in all analyzed populations. In addition, there was a decrease in B lymphocytes, activated cytotoxic T, NKT cells and macrophages when compared to tumor-bearing and tumor-free animals, both treated by this drug. Although cyclophosphamide also reduced the cells in the peritoneum, it was verified that the functional capacity of these cells was not affected, since there was a high production of H2O2 after stimulation with PMA. These results allow us to conclude that the protocol characterized in this study can be used as an efficient pre-clinical model of immunosuppression in animals with or without tumor, considering its effects on the cellularity of lymphoid organs and peripheral tissues.Item DIETA CETOGÊNICA INDUZ ALTERAÇÕES METABÓLICAS E HEPÁTICAS EM ANIMAIS OBESOS(Universidade Federal do Maranhão, 2022-03-22) CARVALHO, Suena Cristina Rodrigues de; PINTO, Bruno Araújo Serra; http://lattes.cnpq.br/2118005601454216; CAPPELLI, Ana Paula Gameiro; http://lattes.cnpq.br/3208933962036057; CAPPELLI, Ana Paula Gameiro; http://lattes.cnpq.br/3208933962036057; PINTO, Bruno Araújo Serra; http://lattes.cnpq.br/2118005601454216; SAMPAIO, Helena Cristina de Lima Barbosa; http://lattes.cnpq.br/3239121044487167; FLISTER, Karla Frida Torres; http://lattes.cnpq.br/4709155952345142; ZANCHI, Nelo Eidy; http://lattes.cnpq.br/2360408543467916Background: Obesity is a metabolic disorder with increasing prevalence at a global level, becoming one of the biggest public health problems today. In this context, nutritional interventions aimed at controlling this disease assume an important relevance. The ketogenic diet (KD) is a diet composed of high levels of fat and low carbohydrates and referred to as efficient for rapid weight loss. Despite its wide use, the effects of this diet on metabolism and liver function are still controversial. Objective: To evaluate the subchronic effects of the ketogenic diet (KD) on the general metabolism and liver function of mice with obesity and other metabolic disorders. Methods: Swiss male mice after weaning were divided into two groups: obese group that received cafeteria diet (CAF) and control group (CTR) that received standard diet. After 12 weeks of follow-up, the animals were divided into 5 groups: CTR/CTR group, which continued receiving CTR chow; CTR/DC group, which replaced the CTR diet with DC; CAF/CAF group, which continued with the diet cafeteria; CAF/CTR group, which replaced the CAF diet with CTR; and CAF/DC group, which replaced the CAF ration with DC. The groups were followed for an additional 8 weeks. During the entire experimental period, measurements of body weight, food intake, glycemic and lipid levels, evaluation of transaminases, degree of glucose tolerance and insulin resistance were performed. After euthanasia, blood was collected for final biochemical and insulin and liver measurements to evaluate oxidative stress and histopathological profile. Results: The CAF diet (CAF/CAF group) was efficient in inducing an obesogenic profile marked by central obesity, dyslipidemia, hyperglycemia, glucose intolerance, insulin resistance, liver damage, oxidative stress and hepatic steatosis. And the administration of DC to previously obese animals (CAF/DC group) was not able to change this dysfunctional profile, being able to control only glycemic levels. Likewise, the administration of DC diet to control animals (CTR/DC group) also led to the establishment of metabolic and hepatic disorders, such as the mentioned groups above. On the other hand, replacing the CAF diet with CTR (CAF/CTR group) was efficient in reversing all these parameters. Conclusion: Our dataset suggests that CD intervention does not lead to weight loss, it establishes metabolic dysfunctions in healthy individuals and worsens it in obese individuals and leads to the development of non-alcoholic fatty liver disease. Replacing a hypercaloric diet with a normocaloric one is more effective in controlling metabolic disorders.Item Efeito antinociceptivo e anti-inflamatório do carvacrol em modelo experimental de osteoartrite em ratas(Universidade Federal do Maranhão, 2022-11-12) ATAIDE, Ana Caroline Santos; CARTÁGENES, Maria do Socorro de Sousa; http://lattes.cnpq.br/3013333572719007; CARVALHO, Rafael Cardoso; RIBEIRO, Rachel Melo; http://lattes.cnpq.br/4752952470368965; FRANÇA, Lucas Martins; http://lattes.cnpq.br/9196798135350419; OLIVEIRA, Renata Mondego de; http://lattes.cnpq.br/8389094419884160; CARVALHO, Rafael Cardoso; http://lattes.cnpq.br/3863794712744490ABSTRACT Osteoarthritis (OA) is a chronic degenerative osteoarticular disease characterized by progressive degradation of joint cartilage, associated with inadequate tissue repair and inflammation. Treatment with standard drugs is quite expensive and associated with adverse effects due to the prolonged use of these drugs, it increases the need to search for new therapeutic options, and among them, chemical compounds extracted from plants are a promising alternative. Among these components, carvacrol stands out, which has numerous biological properties already described in the literature. This work aimed to evaluate the antinociceptive and anti-inflammatory activity of carvacrol in an experimental model of osteoarthritis in rats. To perform this study, 35 rats of the Rattus norvegicus species (Albinus variety), Wistar lineage, adult (60 days old) used up, divided into seven groups, namely: Healthy Group; Sham Group; Negative Control Group: OA induction and without treatment; Carvacrol 20mg group: with OA induction and treated with carvacarol 20 mg/kg, 1x a day; Carvacrol 40mg group: with OA induction and treated with carvacarol 40 mg/kg, 1x a day; Association Group: with OA induction and treated with a combination of carvacrol 10 mg/kg + indomethacin 1.25 mg/kg; Indomethacin Group (Positive Control): animals with OA induction and treated with Indomethacin 2.5 mg/kg, 1x a day. On days 4, 7, 14, 21 and 28 after OA induction and treatment, clinical and behavioral evaluations (motor activity/forced walking, quantification of spontaneous pain and evaluation of mechanical allodynia, and analysis of disability/weight distribution in the hind legs and mechanical hyperalgesia) were performed. In addition, radiological examinations on the 21st day of treatment held up. The animals were treated by gavage and, at the end of the 28-day experimental period, after euthanasia, peripheral blood was collected for hematological and biochemical analysis. The results showed that carvacrol at a dose of 40 mg/kg and its association with indomethacin in the tests carried out had an antinociceptive effect. The pro-inflammatory activity with TNF-α attenuated the production of the TNF-α level in the carvacrol 40 mg/kg and combination groups. In the IL-10 analysis, the indomethacin group, compared with the carvacrol 40 mg/kg groups and the association group, did not show statistical significance, which in this analysis may show similar efficiency to the standard treatment used. In conclusion, the carvacrol 40 mg/kg and association groups showed analgesic and anti-inflammatory activity in the proposed experimental model.Item EFEITO DO EXERCÍCIO ISOMÉTRICO EQUALIZADO EM DENSIDADE SOBRE AS RESPOSTAS HEMODINÂMICAS DE IDOSAS HIPERTENSAS.(Universidade Federal do Maranhão, 2018-03-16) LEITE, Thiago Gomes; LEITE, Richard Diego; 056.304.766-61; http://lattes.cnpq.br/9102502940689012; LEITE, Richard Diego; 056.304.766-61; http://lattes.cnpq.br/9102502940689012Introduction. Isometric exercise has been shown to have great potential in reducing blood pressure, but mainly for the hypertensive elderly population, the effects of training variables, such as density, are still controversial. Objective: To compare the effects of two different isometric exercise protocols, equalized in density, on the acute cardiovascular responses of elderly hypertensive patients. Material and Method: Thirteen (13) hypertensive elderly women participated in the study, who performed two exercise protocols equalized in density. Protocol A: 4 series of 2 minutes, intensity of 30% of 1RM and interval of 2 minutes between each series (protocol A); and Protocol B which corresponded to: 16 series of 30 seconds, intensity of 30% of 1RM and interval of 24 seconds between each series. The following variables were analyzed: systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), heart rate (HR), subjective perception of effort (PSE) and nitrate and nitrite concentration. Results: An increase in SBP, MAP, FC and DP was observed at the post-momentum compared with the pre-exercise moment. In the comparison of the responses between the two protocols, a higher increase in SBP, DBP and HR was observed in protocol B. It was not possible to observe a hypotensive effect immediately after, at 1 hour and / or 24 hours after the exercise sessions. In relation to heart rate variability, in the frequency domain, the HF (High Frequency) showed a reduction after the B protocol, and in the time domain, the RMSSD presented reduction after the two protocols. The concentration of nitrite and nitrate did not change significantly after isometric exercise in any of the protocols. Conclusion: The isometric exercise of lower pause presented greater hemodynamic responses, although none of the protocols presented hypotensive effect. Factors related to metabolism such as nitrate and nitrite concentration, as well as factors related to the autonomic system did not present significant variations suggestive of hypotensive effect.Item EFEITO DO EXTRATO HIDROALCOÓLICO DAS FOLHAS DE Syzygium cumini (L.) SKEELS E DOS SEUS COMPOSTOS MAJORITÁRIOS SOBRE BACTÉRIAS PRESENTES NA MICROBIOTA INTESTINAL E SOBRE CAMUNDONGOS INFECTADOS POR Escherichia coli 042(Universidade Federal do Maranhão, 2020-12-13) SILVA, Iandeyara Savanna Carneiro da; PAES, Antonio Marcus de Andrade; http://lattes.cnpq.br/2310501964710274; ABREU JÚNIOR, Afonso Gomes; http://lattes.cnpq.br/4394413983541820; SILVA, Luís Cláudio Nascimento da; http://lattes.cnpq.br/6016850820500623; GUERRA, Rosane Nassar Meireles; http://lattes.cnpq.br/2316192786452127; SILVA, Ludmila Bezerra da; http://lattes.cnpq.br/3013556982258366; ABREU JÚNIOR, Afonso Gomes; http://lattes.cnpq.br/4394413983541820The intestinal microbiota (IM) is seen as a key regulator in the physiology and pathophysiology of its hosts, demonstrating a relationship with the regulation of fat storage, obtaining energy, as well as an intimate connection with the intestinal lymphoid tissue and its existing immune network. The agents that have been extensively studied in recent years as potential therapies in the IM scenario are the polyphenols found in plants, such as Syzygium cumini (L.) Skeels, also known as purple olives, jambolão or jamelão. Thus, this study aimed to investigate the effect of the hydroalcoholic extract of the leaves of Syzygium cumini (L.) Skeels and the main compounds present in it on bacteria that make up the intestinal microbiota. For this purpose, the 96-well plate microdilution technique was used to determine the Minimum Inhibitory Concentration (MIC) of the hydroalcoholic extract of S. cumini leaves (EHSC) on Escherichia coli, Staphylococcus aureus, Lactobacillus paracasei and Enterococcus faecalis strains, bacteria belonging or with potential to compose IM. From the result obtained in the MIC, the plating of 10 μl aliquots of the wells was performed on plates containing Mueller Hinton agar to determine the Minimum Bactericidal Concentration (CBM), from the visualization of growth or not of bacterial colonies. In addition, the cytotoxicity test was performed from the cell viability test with MTT using HT29 cells and the in vivo colonization of Swiss mice with the E. coli 042 bacterium, and subsequent treatment with the EHSC for analysis and comparison of results. The MIC result was 3.12 mg / mL showing bacteriostatic effect on E. coli 042, E. coli HB101 and S. aureus ATCC 25923 and E. faecalis 29212, and 1.5 mg / mL on L. paracasei. The result of CBM showing a bactericidal effect was 6.25mg / mL on these same bacteria mentioned, with the exception of L. paracasei, which presented a value of 3.1 mg / mL. As for the isolated compounds of the EHSC: myricetin, quercetin and gallic acid, the MIC was 5mg / mL, 0.25mg / mL and 1.25mg / mL, respectively, over S. aureus and L. paracasei. For E. coli 042 and E. faecalis it was 5 mg / mL, 0.25 mg / mL and 0.31 mg / mL, for the same compounds, and 2.5 mg / mL, 0.12 mg / mL and 0.62 mg / ml on E. coli HB101. In addition, gallic acid potentiated the effect of myricetin and quercetin, reducing the value of their MICs on E. coli 042 and L. paracasei when the three isolates were combined. However, all of these values were bacteriostatic, since when the CBM test was performed, none of the concentrations used showed a bactericidal effect. It was possible to observe that there was no toxicity of any of the substances used here on the HT29 cells or on the organs of the evaluated animals. In the animal colonization test, the S. cumini EH decreased the efficiency of E. coli 042 colonization from the 6th day on in the animals. Therefore, it was possible to demonstrate the potential modulatory effect of EHSC, since it presents different values on different bacteria, as well as the synergy of its main compounds, in addition to the possibility of avoiding negative consequences in a possible pathogenic bacterial colonization by decreasing the capacity of this colonization.Item Estudo da prevalência de alterações estomatológicas e o perfil da condição de saúde bucal dos pacientes que vivem com HIV/AIDS, atendidos nas unidades de referência em São Luís – MA(Universidade Federal do Maranhão, 2008-03-26) CARVALHO, Célia Cristina Vieira; ALVES, Maria Teresa Seabra Soares de Britto e; 084261711-68; http://lattes.cnpq.br/3910533965773430; ALVES, Maria Teresa Seabra Soares de Britto e; 084261711-68; http://lattes.cnpq.br/3910533965773430; THOMAZ, Érika Bárbara Abreu Fonseca; http://lattes.cnpq.br/3644251156905353; SILVA, Raimundo Antônio da; http://lattes.cnpq.br/4033051215014128; RIBEIRO, Cecília Cláudia Costa; http://lattes.cnpq.br/4033051215014128This study proposes to show the prevalence of oral changes in people who are living with HIV / aids who have been attending the unit of reference in Sao Luis do Maranhão, in the period April to August 2007. Furthermore, the profile was also checked the condition of oral health of these people. The main results showed that the largest number of people living with HIV / aids is male (58.6%), and most are living with the disease between one and five years. Also the majority is being followed (97.7%), receiving anti-retroviral (80.5%) three (47.5%). Only 36.7% of people who are living with HIV / aids discussed at two units of reference where the research was conducted, showed at some point some type of oral amendment, which 36.2% represents the largest number of change in form bubbles, small (61.7%), the largest percentage (53.2%) reported not feel pain. The largest percentage of examined showed need of some devzntal treatment (54.6%), while 98.4% of people examined when questioned, said brushing your teeth at least three times a day. It was found that the largest number of people living with HIV and aids by being in ambulatory monitoring shows lower prevalence in oral amendments. Those who had any injury was the biggest percentage hipercromática spots at the edge side of the tongue (33.3%) and its profile of oral health is therefore similar to that of the population that does not live with HIV and aids.Item Investigação da atividade anti-agregante plaquetária in vitro de peptídeos inibidores da dissulfeto isomerase protéica - etapa 2(Universidade Federal do Maranhão, 2016-12-06) SOUSA, Hiran Reis; PAES, Antônio Marcus de Andrade; 453.491.113-34; SANTOS, Ana Paula Silva de Azevedo dos; 717.120.543-68Recent researches have emphasized the importance of redox mechanisms for platelet function modulation. The platelet surface contains a large variety of integrin receptors and other molecules presenting functional thiol groups in their structures, which are potential targets for redox regulation. Among these various thiol-containing proteins, integrin αIIbβ3 stands out for being the convergence path of platelet activation induced by various agonists. Activation of αIIbβ3 integrin is catalyzed by protein disulfide isomerase (PDI) through an essential conformational change leading to the exposure of fibrinogen-binding site. Thus, PDI has been shown to be an important target for the development of antiplatelet drugs. In recent years, many studies have described substances from plan (DE A. PAES et al., 2011), as well as synthetics that are capable of inhibiting PDI. In a previous study of our research group has shown that the synthetic peptide CxxC, which contains the redox motif of PDI in its original sequence CGHC, inhibited reductase activity of this enzyme, effect not observed with AxxA peptide, whose cysteines were replaced with alanine and Scr peptide, which contains the same aminoacids from CxxC peptide, but under random sequence. It has been also demonstrated that CxxC peptide was the only to reduce by 30% ADP-induced aggregation (5μM) in platelet rich plasma, an effect apparently mediated by the association of CxxC and PDI at platelet surface. Thus, in this work, we further assessed the effects of CxxC and its control peptides on platelet aggregation. Washed human platelets were incubated with CxxC peptide at concentrations of 3, 6 and 10 μM, resulting in a dose-dependent inhibition of maximum aggregation activated by thrombin (0.02 U/mL) at 25, 60 and 74%, respectively with IC50 of 6.13 ± 1.09 μM. The presence of control peptides did not produce any inhibitory effect. CxxC peptide also reduced the activation of αIIbβ3 integrin at platelet surface, but did not affect the expression of the markers CD 62-P and CD 63. Control peptides did not alter the expression of these markers. Analysis by mass spectrometry of the interaction of recombinant human PDI with the peptide showed that only CxxC peptide associated with the redox Cys400 of a’ motif of PDI, which has been considered essential for platelet aggregation. Together, these results demonstrate that CxxC peptide reduces platelet aggregation by association with PDI and can be further used as a model for the development of new antithrombotic drugs.Item Padronização de extratos para a obtenção de produto fitoterápico antifúngico de Origanum vulgare L.(Universidade Federal do Maranhão, 2021-01-28) BEZERRA , Samara Araújo; COUTINHO, Denise Fernandes; http://lattes.cnpq.br/7346399893912346; COUTINHO, Denise Fernandes; http://lattes.cnpq.br/7346399893912346; NEIVA, Flávia Maria Mendonça do Amaral; http://lattes.cnpq.br/9334507801916334; MESQUITA, Ludmilla Santos Silva de; http://lattes.cnpq.br/9775085514370412; MONTEIRO NETO, Valério Monteiro; http://lattes.cnpq.br/5476942147520772Among the main infectious diseases, candidiasis is considered opportunistic, being the most common disease caused by fungi. Resistance to antifungal therapies is a public health problem and has been caused by the extensive prescription of these drugs associated with low adherence to treatment, in addition to repeated or prolonged therapy. In order to discover effective therapeutic alternatives, medicinal plants are sought for compounds that can be used in this context, exploring therapeutic benefits from the wide variety of biologically active metabolites. This work aimed to standardize the extract of the leaves of the species Origanum vulgare L. (Lamiaceae - oregano), to subsidize the elaboration of a product for vaginal use, contributing to obtain alternatives for the treatment of vulvovaginal candidiasis. Dry leaves of Origanum vulgare L. were collected at the Federal University of Maranhão, city of São Luís, Maranhão and submitted to three different extraction methods: maceration, percolation and soxhlet, using hydromodules for each extraction: 1: 8, 1:10 and 1:12. The extracts obtained were evaluated qualitatively using phytochemical tests, regarding the content of flavonoids and polyphenols and were subjected to evaluation of their antifungal potential against strains of Candida. Extraction by Sohxlet, hydromodule 1:10 showed 41.11% yield, while phytochemical screening showed variations in constituents, presence of polyphenols and tannins in different concentrations for the tested extracts, also indicating differentiated antioxidant activity that may corroborate with the mechanism antifungal. Liquid chromatography showed nine main peaks, including rosmarinic acid, defined as an analytical marker. All extracts showed fungistatic activity. From the results, in order to standardize the extraction process, an extract obtained by soxhlet 1:10 was chosen, which presented better phytochemical and antifungal results, in addition to superior yield. With the standardized extract, a pilot bioproduct was prepared, in the pharmaceutical form of eggs at 5%, having demonstrated good stability for 20 days. Thus, it was demonstrated the process of obtaining an extract of the leaves of Origanum vulgare in addition to stable formulations for therapeutic use, therefore, a promising alternative in the treatment of vulvovaginal candidiasis.Item PADRONIZAÇÃO E USO DA ARMADILHA LUMINOSA SILVA À BASE DE LEDS (LIGHT-EMITTING DIODES) NAS CAPTURAS DE MOSQUITOS ANOFELINOS NO ESTADO DO MARANHÃO.(Universidade Federal do Maranhão, 2021-06-10) ARAUJO, Eudimara Carvalho de; SILVA, Francinaldo Soares; http://lattes.cnpq.br/3390673784617972; SILVA, Francinaldo Soares; http://lattes.cnpq.br/3390673784617972; REBÊLO, José Manuel Macário; http://lattes.cnpq.br/1026308157852394; ARAÚJO, Maisa da Silva; http://lattes.cnpq.br/1036895487969696; PINHEIRO, Valéria Cristina Soares; http://lattes.cnpq.br/5443198431811220Malaria is an infectious disease transmitted to man after exposure by the bites of infected anopheline females. For a long time, malaria has been a persistent health problem with high mortality and morbidity occurring mainly in the developing countries. As the transmission cycle of malaria varies worldwide, entomological surveillance is an important component in the fight against malaria. Thus, several collecting tools, such as the Center for Disease and Control and Prevention light trap (CDC), the light trap (HP), the BioGents® Sentinel and Malaria trap (BG-Sentinel; BG-Malaria) have been employed extensively. Such traps have some disadvantages such as high costs, need of chemical odors to attract mosquitoes and the problems of damaging the collected specimens, causing difficulties to species identification. Hence, this research aims at standardizing a passive light trap, the Silva Trap (ST), for the captures of anopheline mosquitoes in peridomicile and forest environments. This light trap was efficiently used for sampling anopheline mosquitoes in pig pens, but its efficiency in peridomicile and forest areas needs to be evaluated. Thus, in the first moment, three LED colors (blue, 470 nm, 15,000 mcd; green, 470 nm, 20,000 mcd; ultraviolet, 395 nm, 3,000 mcd) had their efficiency as light sources for sampling anopheline mosquitoes in the peridomicile settings evaluated. In the second moment, different heights (0,5m; 1,0m; 1,5m; 2,0m) for a better use of the Silva trap were evaluated. In the third moment, the most attractive color and the best height for installing the Silva trap were chosen to capture anopheline mosquitoes in a forest environment. In this later case, HP light traps were used as “control”. In total, 9,009 specimens and nine species were collected: Anopheles triannulatus s.l, An. argyritarsis, An. goeldii, An. rangeli, An. galvaoi, An. evansae, An. darlingi, An. mediopunctatus and An. neomaculipalpus. In the first moment, a total of 3,750 specimens was captured and the green LED attracted 48.21% (58.32± 13.93followed by the bleu LED (44.93%, 56.17 ± 14.44) and ultraviolet (6.85%, 9.88 ± 1.74). In the second moment, 2,955 anopheline mosquitoes were captured, with the following results: 0,5m (17.63%, 30.65± 4.79/media ± SEM), 1,0 m (28,29%, 44± 7.61), 1,5 m (32,04%, 47.35± 8.53) and 2,0 m (22,03%, 34.26± 7.60). In the last moment, the Silva trap, with green LED, attracted 38,88% (59.73± 6.69), followed by the HP with green LED 36,37% (55.87± 11.43) and HP with incandescent light 24,73% (38±6.54). The Silva trap with the green LED and deployed at 1,5m above soil was the most efficient mode of operation of the Silva light trap, with a significant statistical result only observed between the Silva trap with a green LED and the HP with incandescent light, demonstrating that the use of the Silva trap and LEDs in the monitoring and, possibly, controlling programs should be considered.Item Potencial modulador dos metabólitos da semente e polpa de Euterpe oleracea Mart. sobre a via Orai1/STIM1/NFAT de linfócitos T: uma abordagem in silico(Universidade Federal do Maranhão, 2023-10-27) SOUSA, Nágila Caroline Fialho; PEREIRA, Paulo Vitor Soeiro; http://lattes.cnpq.br/7281767698416958; SOEIRO, Paulo Vitor; http://lattes.cnpq.br/7281767698416958; FRANÇA, Lucas Martins; http://lattes.cnpq.br/9196798135350419; RIBEIRO, Rachel Melo; http://lattes.cnpq.br/4752952470368965; PESSOA, Débora Luana Ribeiro; http://lattes.cnpq.br/2537676284852975The species Euterpe oleracea Mart, known as açaí and native to the Amazon region, is valued for its nutritional properties and health benefits, including antioxidant, anti-inflammatory and immunomodulatory activities. However, the full potential action of the secondary metabolites of this plant species on the immune system is not yet known. In this context, the present work aimed at the in silico evaluation of major compounds from açaí seed extract and pulp in the Orai1/STIM1/NFAT pathway of T lymphocyte activation. The compounds procyanidin C1 (PCC1), Epigallocatechin-3,3-di-O-Gallate (EGCdiG), patulithrin (PAT) and cyanidin (CY) were selected for computational analysis. The structures of the compounds were obtained from PubChem and used to evaluate the physicochemical characteristics, prediction of biological activity spectra, toxicological data, theoretical oral bioavailability, interaction profile of compounds with pharmacological targets, and interaction with proteins of the pathway of interest through molecular docking. All compounds evaluated showed relevant physicochemical properties, showing promise for some route of administration in the radar bioavailability analysis, with emphasis on cyanidin and patulithrin with the best oral bioavailability. According to pharmacokinetic parameters, these two compounds showed potential for absorption in the gastrointestinal tract and penetration through the skin. PASS predictions of biological activity spectra revealed that all compounds have potential for various activities, including peroxidase inhibition, free radical scavenging, antioxidant effects, and regulation of the immunoinflammatory response. Furthermore, CY and PAT exhibited low toxic potential in hepatotoxicity, cytotoxicity and mutagenicity. Molecular docking analysis demonstrated that CY (-7.0 kcal/mol) and PAT (-8.3 kcal/mol) bind to the Orai1 regulatory site (Ala282 and Phe171, respectively), while EGCdiG (-7.0 kcal/mol) and CY (-6.0 kcal/mol) exhibited affinity for the STIM1 activation/regulation site (Gly22 and Asp32, respectively). These results demonstrate that the selected compounds, mainly cyanidin and patulithrin, present characteristics that validate their future use as modulating agents of T lymphocyte activity, due to their interaction with proteins of the Orai1/STIM1/NFAT pathway.Item Qualidade de vida, dor, depressão e ansiedade em pacientes com câncer de reto tratados cirurgicamente(Universidade Federal do Maranhão) SANTOS, Letácio José Freire; GARCIA, João Batista Santos; 135570488-02; http://lattes.cnpq.br/0424234103760462; GARCIA, João Batista Santos; 135570488-02; http://lattes.cnpq.br/0424234103760462; TORRES, Orlando Jorge; http://lattes.cnpq.br/6448439573128558; GOMES, Josenília Maria Alves; http://lattes.cnpq.br/7813338472725202; AMORIM, EliasObjective: To evaluate Life Quality, pain, anxiety and depression in patients treated for medium and lower rectum cancer, submitted to surgical intervention for healing aims. Methods: A descriptive cross-sectional study. Eighty-eight patients with medium and lower rectum cancer, submitted to surgical intervention for healing aims were selected, and enrolled from January 2006 to December 2010. Forty-seven patients died within the study period, and the other 41 were studied. Question forms EORTC QLQ-C30 and EORTC QLQ-CR38 were used to assess life quality. Pain evaluation was carried out using the Visual Analogical Scale, depression and anxiety were assessed through Depression Inventories and Beck’s Anxiety, respectively. The correlation between pain intensity, depression and anxiety was carried out, and between these and the EORTC QLQ-C30 General Scale for Health status and Overall Life Quality, as well as the EORTC QLQ-CR38 functional and symptom scales. Results: Of the 41 patients of the study, 52% presented pain, depression in 47%, and anxiety in 39%. There was a marking positive correlation between pain intensity and depression. There was a moderate negative correlation between depression and general health status, and overall life quality as well as pain intensity with the latter. There was a statistically significant negative correlation between future depression perspective and sexual function, and also a strong positive correlation between depression and sexual impairments. A positive correlation between anxiety and gastro-intestinal sexual problems is observed, both statistically significant.Item Resposta imune na infecção mista por Leishmania amazonensis e Leishmania chagasi em macrófagos(Universidade Federal do Maranhão, 2021-08-04) PINHEIRO, Luana Caroline Santos; SILVA, Lucilene Amorim; http://lattes.cnpq.br/9794797427532881; SILVA, Lucilene Amorim; http://lattes.cnpq.br/9794797427532881; SÁ, Joicy Cortez de; http://lattes.cnpq.br/2368453114845145; REIS, Aramys Silva dos; http://lattes.cnpq.br/1040580590566490; PEREIRA, Paulo Vitor Soeiro; http://lattes.cnpq.br/7281767698416958Leishmaniasis is endemic in several regions of the world, being considered a major public health problem. In this context, mixed infection by different Leishmania species in the same individual, especially in areas of overlapping LTA and LV species, may be more prevalent than previously reported, affecting the clinical course, diagnosis and treatment of the disease. In this research, a review was carried out on the panorama of mixed infection, presented as chapter 1, which aimed to identify the work already carried out on this topic. Many case reports and a scarcity of experimental works were identified. But the results of the studies revealed that in a shared environment, different species can show different behaviors and compromise the course of the infection. In chapter 2, the work carried out, which aimed to evaluate the immune response and behavior of parasites in mixed infection by Leishmania amazonensis and Leishmania chagasi. For this, the macrophages were infected by L. amazonensis and L. chagasi, at times of 24 and 48 hours, then the infection rate, production of nitric oxide, hydrogen peroxide, production of inflammatory cytokines, in addition to the morphometric analysis were evaluated. of the parasitophorous vacuoles. The initial results related to the infection rate showed an overlap of L. chagasi in the mixed infection, suggesting the occurrence of an uneven proliferation and competition. Regarding the production of hydrogen peroxide, the mixed infection group showed a production similar to that seen in the single infection by L. chagasi, and this species may be influencing the production of this reactive in the mixed infection. The production of cytokine TNF mixed infection was similar to that observed in the single infection by L. amazonensis, so it is believed that the presence of this species may be relevant for the production of this cytokine in the shared environment. The MCP-1 chemokine was a parameter that showed no difference between the groups, even though it is an important chemokine linked to the recruitment of monocytes and the increase in the number of macrophages during the single and mixed infection. After evaluating the amount of parasitophorous vacuoles, a smaller amount of these structures in the mixed infection group was observed within 48 hours, a result that may be related to the strategy of sharing vacuoles by the parasites, which has already been analyzed in other studies with other species. Regarding the area of the vacuole, an increase in extension was noted only in the group with single infection by L. chagasi, within 48 hours. In view of the results, it was possible to observe that in a shared environment, the influence of species occurs differently, with changes in the behavior of vacuoles and the action of the cytokine TNF- e protozoan in the host and compromise the clinical course of infectionItem Soroconversão tardia do HBeAg em portadores do subgenótipo D4 do vírus da hepatite B(Universidade Federal do Maranhão, 2016-05-20) SOUZA, Marinilde Teles; FERREIRA, Adalgisa de Souza Paiva; 850.818.767-04Introduction: The hepatitis B virus (HBV) present diversity of its genome, which is to be classified in different genotypes and subgenotypes (A-J). It has been demonstrated that different genotypes are related to the natural history of infection. The maintenance of viral replication could be one of the factors related to genotypes. Objectives: To identify the viral replication status of HBV carriers among the subgenótipos A1 and D4. Materials and methods: HBV carriers identified have been studied in two studies involving individuals from the state of Maranhão, northeast,Brazil, which had genotyping and subgenotypes, serology for HBeAg and anti-HBe and certain viral loads. Serological tests were performed by ELISA, HBV – DNA quantification real time PCR and genotyping performed by sequencing. Results: We identified 146 patients. Among these, 136 were subgenotype A1 or D4. It is 85 - A1 (62.5%) and 51 - D4 (37.5%). No difference was found between groups when age was evaluated (42 ± 12 vs 38 ± 17 p=0.11) or gender (male 48.5% vs 51.5% p=00.18). Among the D4 subgenotype carriers had more patients with HBeAg positive (23.5% vs 9.4%, p=0.02) and a higher proportion of patients with viral loads above 20.000 IU / ml (43.1% vs 12.9 % p <0.0001), even when only those with negative HBeAg (25.6% vs 6.5%, p=0.007) when compared with the A1 subgenotype. Conclusion: HBV carriers, subgenotype D4, compared to A1 subgenotype have delayed HBeAg seroconversion and higher levels of HBV – DNA, suggesting that this subgenotype is possibly related toItem USO PROFILÁTICO DE EXTRATO PADRONIZADO DE PRÓPOLIS DE Apis melifera (EPP-AFâ) REDUZ INFLAMAÇÃO NO PARÊQUIMA PULMONAR E AUMENTA A EXPECTATIVA DE VIDA EM CAMUNDONGOS COM SEPSE(Universidade Federal do Maranhão, 2021-06-11) GARBIS, Dimítrius Vidal de Oliveira; BRAGA, Thiare Silva Fortes; http://lattes.cnpq.br/5827678707239821; NASCIMENTO, Flávia Raquel Fernandes do; http://lattes.cnpq.br/9073277157401960; NASCIMENTO, Flávia Raquel Fernandes do; http://lattes.cnpq.br/9073277157401960; SILVA, Andresa Aparecida Beretta e; ABREU JÚNIOR, Afonso Gomes; AZEVEDO, Conceição de Maria Pedrozo e Silva dePropolis is a resinous balsamic material, used to protect the hive against insects and microorganisms. In addition to the antimicrobial activities of propolis, its anti-inflammatory and immunomodulatory effect stands out. The aim of this study was to evaluate the effect of treatment with Standardized Propolis Extract (EPP-AF®) on sepsis induced by ligation and cecal perforation (CLP). Initially, for survival assessment, Swiss mice were separated into 5 groups: SHAM (without cecal perforation), CLP (Saline + CLP), CLP + ATB (Antibiotic + CLP), EPP10 (EPP-AF® 10mg / kg + CLP), EPP100 (EPP-AF® 100mg / kg + CLP). Subcutaneous administration of antibiotics or EPP-AF® was performed 6 hours before the CLP procedure. Animal survival was assessed every 12 hours for 5 days. The data showed an increase of 51%, 16% and 25% in the life expectancy of animals in the CLP + ATB, EPP10 and EPP100 groups, respectively, in relation to the CLP group. After assessing survival, the CLP and EPP100 groups were repeated to assess immunophysiological parameters. After 12 hours of CLP, the animals were euthanized, and the biological material was collected for proper analysis. The data demonstrated that the single administration of EPP-AF®, prophylactically, did alter neither the colony-forming units in the peritoneum, blood and lung, nor the peripheral blood, marrow and coagulogram cell count (number of platelets, Prothrombin time and Activated partial thromboplastin time). However, the peritoneum cell count as well as the inflammatory infiltrate and hemorrhage in the lung tissue were lower in the animals that received EPP-AF®. Thus, it was possiple to conclude that EPP-AF® at a concentration of 100mg / kg, when used in a prophylactically way, promoted better preservation of the lung parenchyma, which may explain the increase in lifespan presented by mice with CLP-induced sepsis.