USO PROFILÁTICO DE EXTRATO PADRONIZADO DE PRÓPOLIS DE Apis melifera (EPP-AFâ) REDUZ INFLAMAÇÃO NO PARÊQUIMA PULMONAR E AUMENTA A EXPECTATIVA DE VIDA EM CAMUNDONGOS COM SEPSE

Abstract

Propolis is a resinous balsamic material, used to protect the hive against insects and microorganisms. In addition to the antimicrobial activities of propolis, its anti-inflammatory and immunomodulatory effect stands out. The aim of this study was to evaluate the effect of treatment with Standardized Propolis Extract (EPP-AF®) on sepsis induced by ligation and cecal perforation (CLP). Initially, for survival assessment, Swiss mice were separated into 5 groups: SHAM (without cecal perforation), CLP (Saline + CLP), CLP + ATB (Antibiotic + CLP), EPP10 (EPP-AF® 10mg / kg + CLP), EPP100 (EPP-AF® 100mg / kg + CLP). Subcutaneous administration of antibiotics or EPP-AF® was performed 6 hours before the CLP procedure. Animal survival was assessed every 12 hours for 5 days. The data showed an increase of 51%, 16% and 25% in the life expectancy of animals in the CLP + ATB, EPP10 and EPP100 groups, respectively, in relation to the CLP group. After assessing survival, the CLP and EPP100 groups were repeated to assess immunophysiological parameters. After 12 hours of CLP, the animals were euthanized, and the biological material was collected for proper analysis. The data demonstrated that the single administration of EPP-AF®, prophylactically, did alter neither the colony-forming units in the peritoneum, blood and lung, nor the peripheral blood, marrow and coagulogram cell count (number of platelets, Prothrombin time and Activated partial thromboplastin time). However, the peritoneum cell count as well as the inflammatory infiltrate and hemorrhage in the lung tissue were lower in the animals that received EPP-AF®. Thus, it was possiple to conclude that EPP-AF® at a concentration of 100mg / kg, when used in a prophylactically way, promoted better preservation of the lung parenchyma, which may explain the increase in lifespan presented by mice with CLP-induced sepsis.